蒋鹏飞, 彭俊, 谭涵宇, 欧阳云, 覃艮艳, 彭清华. 密蒙花颗粒对去势雄兔泪腺细胞炎症因子TGF-β1，NF-κB，IL-10和IL-12表达的影响[J]. Digital Chinese Medicine, 2019, 2(2): 97-104. DOI: 10.1016/j.dcmed.2019.09.004
引用本文: 蒋鹏飞, 彭俊, 谭涵宇, 欧阳云, 覃艮艳, 彭清华. 密蒙花颗粒对去势雄兔泪腺细胞炎症因子TGF-β1，NF-κB，IL-10和IL-12表达的影响[J]. Digital Chinese Medicine, 2019, 2(2): 97-104. DOI: 10.1016/j.dcmed.2019.09.004
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JIANG Peng-Fei, PENG Jun, TAN Han-Yu, OU-YANG Yun, QIN Gen-Yan, PENG Qing-Hua. Effects of Buddlejae Flos Granules on Inflammatory Factors TGF-β1, NF-κB, IL-10 and IL-12 in Lacrimal Gland Cells of Castrated Male Rabbits[J]. Digital Chinese Medicine, 2019, 2(2): 97-104. DOI: 10.1016/j.dcmed.2019.09.004
Citation: JIANG Peng-Fei, PENG Jun, TAN Han-Yu, OU-YANG Yun, QIN Gen-Yan, PENG Qing-Hua. Effects of Buddlejae Flos Granules on Inflammatory Factors TGF-β1, NF-κB, IL-10 and IL-12 in Lacrimal Gland Cells of Castrated Male Rabbits[J]. Digital Chinese Medicine, 2019, 2(2): 97-104. DOI: 10.1016/j.dcmed.2019.09.004
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密蒙花颗粒对去势雄兔泪腺细胞炎症因子TGF-β1，NF-κB，IL-10和IL-12表达的影响

Effects of Buddlejae Flos Granules on Inflammatory Factors TGF-β1, NF-κB, IL-10 and IL-12 in Lacrimal Gland Cells of Castrated Male Rabbits

    摘要
  • 摘要:
    目的观察密蒙花颗粒对去势雄兔泪腺细胞炎症因子TGF-β1、NF-κB、IL-10和IL-12表达的影响。
    方法将30只新西兰长耳白兔随机平均分为5组，A：空白组；B：模型组；C：密蒙花颗粒组；D：安慰剂组；E：睾酮组。除A组外，所有兔子均行双侧睾丸及附睾切除术造成干眼模型。除A、B组外，从造模后第3天开始C组予密蒙花颗粒，按100 mg/kg 灌胃，3次/日；D组予生理盐水灌胃；E组按 0.5 mL/kg 在大腿肌肉处注射丙酸睾酮注射液，每3天注射一次。在造模前1天和造模后第30天行基础泪液分泌试验（SIT）和泪膜破裂时间（BUT）检测。造模30天后，将所有兔子处死，摘取泪腺组织进行免疫组化染色，检测各组兔泪腺组织中TGF-β1、NF-κB、IL-10和IL-12的平均光密度，观察泪腺组织结构。
    结果SIT及 BUT检测结果显示：与A组相比，B、D组差异均有统计学意义（P < 0.05）；C、E组差异均无统计学意义（P > 0.05）。术后各组炎症因子TGF-β1、NF-κB、IL-10和IL-12比较：术后 B、D组比较，差异无统计学意义（P > 0.05）；B、D组与其他各组比较，差异均有统计学意义（P < 0.01）；A、C、E组间比较，差异无统计学意义（P > 0.05）。
    结论密蒙花颗粒对泪腺细胞中炎症因子TGF-β1、NF-κB、IL-10和IL-12的表达具有抑制作用，并通过抑制泪腺细胞中炎症因子TGF-β1、NF-κB、IL-10和IL-12的表达减轻泪腺组织的炎症反应，达到治疗干眼的目的。

     

    Abstract:
    ObjectiveTo observe the effects of Buddlejae Flos (Mi Meng Hua, 密蒙花) granules on the expression of TGF-β1, NF-κB, IL-10 and IL-12 in lacrimal gland cells in castrated male rabbits.
    MethodsA total of 30 New Zealand male white rabbits were randomly divided into 5 groups. Group A: blank group; group B: model group; group C: Buddlejae Flos (Mi Meng Hua, 密蒙花) granules group; group D: placebo group; group E: testosterone group. Except for the group A, all rabbits were treated with bilateral testicular and epididymis resection before intervention. Rabbits in group C were administered with Buddlejae Flos (Mi Meng Hua, 密蒙花) granules (100 mg/kg), 3 times per day; Rabbits in group D were administered with normal saline, 3 times per day. Rabbits in group E were injected with testosterone propionate (0.5 mL/kg) in the thigh muscle, every 3 days. Schirmer I test (SIT) and break-up time (BUT) were measured on 1st day before modeling and 30th day after modeling. After 30 days of intervention, all rabbits were sacrificed. The removal lacrimal gland was stained in immunohistochemica staining. The expression of TGF-β1, NF-κB, IL-10 and IL-12 in lacrimal gland tissue of each group was detected, and the structure of lacrimal gland was observed.
    ResultsThe results of SIT and BUT showed that there was significant difference between group B and D compared with group A (P < 0.05). There were no significant differences between group C and E (P > 0.05). There were no significant differences in the comparison of inflammatory factors TGF-β1, NF-κB, IL-10 and IL-12 between group B and D (P > 0.05). There were significant differences between group B and D compared with other groups (P < 0.01). There was no significant difference between group A, C and E (P > 0.05).
    ConclusionsBuddlejae Flos (Mi Meng Hua, 密蒙花) granules have an inhibitory effect on the expression of TGF-β1, NF-κB, IL-10 and IL-12 in castrated male rabbit lacrimal gland cells, and alleviate the inflammation of lacrimal gland tissue by inhibiting the expression of TGF-β1, NF-κB, IL-10 and IL-12 in lacrimal gland tissue, so as to treat dry eye.

     

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