李伟, Muhammad Daniyal, 谢青玲, 刘杨, 谢谦, 李斌, 彭彩云, 王炜. 土家药白三七三萜皂甙及其对Hep G2和BGC-823细胞系的细胞毒性[J]. Digital Chinese Medicine, 2019, 2(2): 117-124. DOI: 10.1016/j.dcmed.2019.09.006
引用本文: 李伟, Muhammad Daniyal, 谢青玲, 刘杨, 谢谦, 李斌, 彭彩云, 王炜. 土家药白三七三萜皂甙及其对Hep G2和BGC-823细胞系的细胞毒性[J]. Digital Chinese Medicine, 2019, 2(2): 117-124. DOI: 10.1016/j.dcmed.2019.09.006
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LI Wei, Muhammad Daniyal, XIE Qing-Ling, LIU Yang, XIE Qian, LI Bin, PENG Cai-Yun, WANG Wei. Triterpenoid Saponins from Tu Jia Ethnomedicine Bai San Qi and Their Cytotoxicity on Hep G2 and BGC-823 Cell Lines[J]. Digital Chinese Medicine, 2019, 2(2): 117-124. DOI: 10.1016/j.dcmed.2019.09.006
Citation: LI Wei, Muhammad Daniyal, XIE Qing-Ling, LIU Yang, XIE Qian, LI Bin, PENG Cai-Yun, WANG Wei. Triterpenoid Saponins from Tu Jia Ethnomedicine Bai San Qi and Their Cytotoxicity on Hep G2 and BGC-823 Cell Lines[J]. Digital Chinese Medicine, 2019, 2(2): 117-124. DOI: 10.1016/j.dcmed.2019.09.006
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土家药白三七三萜皂甙及其对Hep G2和BGC-823细胞系的细胞毒性

Triterpenoid Saponins from Tu Jia Ethnomedicine Bai San Qi and Their Cytotoxicity on Hep G2 and BGC-823 Cell Lines

    摘要
  • 摘要:
    目的生活在中国中南部的土家族人民传承并应用的土家医药是一种独特的医疗体系。在土家药中，白三七（Panax japonicus C. A. Mey.）被认为是一种用于治疗虚劳和风湿病的有效且珍贵的药材。本文期望为土家药的应用提供更多的物质证据。
    方法柱色谱和制备型高效液相用于分离和纯化；1H-NMR，13C-NMR，1H-1H COSY，HSQC和HMBC核磁共振谱用于结构鉴定；MTT法用于细胞毒性评价。
    结果12个已知化合物从白三七中分离得到，这些化合物的结构是根据它们的核磁共振谱鉴定的，所有12种化合物均为三萜皂苷，其中5个化合物是齐墩果酸型，其余7个是达玛烷型；本研究对11个化合物进行了Hep G2和BGC-823细胞系的细胞毒性测试，其中3个化合物显示了相对显著的细胞毒性。
    结论12个已知化合物从白三七中分离得到；具有相似骨架的化合物的核磁共振谱显示了特定的规律；其中3个化合物显示了对于Hep G2和BGC-823细胞系相对显著的细胞毒性，当与阳性对照紫杉醇对比时，该结果可评价为毒性较低。

     

    Abstract:
    ObjectiveTu Jia ethnomedicine is a unique medical system inherited and adhibited by Tu Jia minority living in central-south China. Panax japonicus C. A. Mey. (Bai San Qi, 白三七) is recognized as an effective and rare medicinal plant to treat weakness, fatigue and rheumatism in Tu Jia ethnomedicine. This paper is to discover more substance evidence for the application of Tu Jia ethnomedicine.
    MethodsColumn chromatography and preparative high performance liquid chromatography (HPLC) was applied for isolation and purification; 1H-NMR, 13C-NMR, 1H-1H COSY, HSQC and HMBC NMR spectra were applied for structure identification; Methyl thiazolyl tetrazolim (MTT) assays were applied for cytotoxicity evaluation.
    ResultsTotally 12 known compounds were isolated by column chromatography and preparative HPLC from rhizomes of Panax japonicus C. A. Mey. (Bai San Qi, 白三七). Structures of these compounds were identified by their NMR spectra. All the 12 compounds were triterpenoid saponins. Five of them were oleanolic acid type, and the remaining 7 were dammarane type. Eleven compounds were assayed for their cytotoxic activity against Hep G2 human liver cancer cell lines and BGC-823 human gastric cancer cell lines. Three of the 11 showed relatively dominant cytotoxicity against these cell lines.
    ConclusionsA total of 12 known compounds have been identified from Panax japonicus C. A. Mey. (Bai San Qi, 白三七); NMR spectra of compounds with similar skeletons showed regular characteristics; 3 compounds showed relatively dominant cytotoxicity against Hep G2 and BGC-823 cancer cell lines, and the result can be valued as weak while setting the taxol as a positive control.

     

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