益气解毒方水提物通过线粒体途径诱导鼻咽癌干细胞凋亡

Yi Qi Jie Du Decoction Inhibits Proliferation and Induces Apoptosis of Nasopharyngeal Carcinoma Stem Cells Through Mitochondrial Apoptosis Pathway

    摘要
  • 摘要:
    目的 研究益气解毒方（YQJDD）水提物对鼻咽癌干细胞（NPC-SCs）增殖和凋亡的影响及机制。
    方法 采用无血清悬浮培养法分离培养NPC-SCs，成球实验鉴定NPC-SCs；CCK-8法检测YQJDD对NPC-SCs细胞增殖的影响；JC-1染色法分析YQJDD对NPC-SCs线粒体膜电位的影响；Western blot检测凋亡相关蛋白（cleaved caspase 3、7、8、9、cleaved PARP、P21、P53、Survivin）的表达。
    结果 YQJDD可抑制NPC-SCs的增殖，且随着药物处理时间和浓度的增加，抑制效果增强（P < 0.05）；YQJDD可降低NPC-SCs细胞线粒体膜电位，诱导细胞凋亡；YQJDD可提高NPC-SCs细胞中cleaved caspase 3、7、9、cleaved PARP、P21、P53的表达，降低Survivin的表达，但对cleaved caspase 8的表达没有影响。
    结论 YQJDD可通过线粒体凋亡途径诱导NPC-SCs细胞凋亡，抑制细胞增殖。

     

    Abstract:
    Objective To assess the effects of Yi Qi Jie Du Decoction (YQJDD) on nasopharyngeal carcinoma stem cells (NPC-SCs) and investigate the underlying mechanism.
    Methods NPC-SCs were collected in serum-free culture system and identified by the sphere formation assay. The effect of YQJDD on the proliferation of NPC-SCs was detected using the Cell Counting Kit-8 assay. The change of mitochondrial membrane potential (ΔΨm) in NPC-SCs following treatment with YQJDD was investigated by JC-1 staining. The levels of apoptosis-associated proteins were examined using western blot analysis.
    Results YQJDD significantly inhibited the proliferation of NPC-SCs in dose- and time-dependent manners. JC-1 staining revealed that YQJDD lowered the ΔΨm of NPC-SCs in a time-dependent manner. After treatment with YQJDD, the expression of cleaved caspase-3, -7 and -9, cleaved poly-ADP ribose polymerase, P21 and P53 were increased, while the expression of Survivin in NPC-SCs was decreased. However, the expression of cleaved caspase-8 remained nearly unchanged.
    Conclusions YQJDD inhibits the growth and induces apoptosis of NPC-SCs via an intrinsic apoptosis pathway.

     

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