电针对糖尿病胃轻瘫大鼠PDGF和线粒体超微结构的影响

魏星; 彭艳; 赵东凤; 肖小娟; 林亚平

doi:10.1016/j.dcmed.2020.03.004

电针对糖尿病胃轻瘫大鼠PDGF和线粒体超微结构的影响

Effect of Electroacupuncture on Platelet-derived Growth Factor and the Ultrastructure of Mitochondria in Rats with Diabetic Gastroparesis

摘要

摘要:
目的观察电针足三里、三阴交、梁门对糖尿病胃轻瘫大鼠血小板源性生长因子(PDGF)和线粒体超微结构的影响。
方法60只SD大鼠随机分入空白对照组(10 只)和造模组(50 只)。造模组采用单次腹腔注射2%链脲佐菌素和8周不规则喂养高脂高糖饲料来建立糖尿病胃轻瘫大鼠模型,每周记录血糖值及症状积分。成功造模后，选取30只造模组大鼠随机分为糖尿病胃轻瘫模型组(10 只)，电针组(10 只)，胃复安组(10 只)。干预结束后，酚红灌胃法测定胃排空率和小肠推进率。免疫荧光法测定Ca²⁺浓度，酶联免疫法(ELISA)测定血清胰岛素和PDGF含量,透射电镜观察线粒体超微结构。
结果（1）干预后，与模型组相比，电针组血糖值和症状积分均显著降低(P < 0.01)。此外，与模型组相比,电针组胃肠推进率显著升高(P < 0.01)，电针组与空白对照组则无明显差别。（2）与空白对照组相比，模型组INS含量明显减少(P < 0.05)，但电针组和胃复安组无明显区别。（3）与模型组相比，电针组和胃复安组大鼠Ca²⁺浓度均明显升高（P < 0.01, P < 0.05）。（4）与空白组相比，模型组PDGF平均光密度值明显升高（P < 0.01）；与模型组比较，电针组PDGF平均光密度值显著升高（P < 0.01）。（5）空白组线粒体丰富，膜及嵴结构清晰；模型组线粒体严重肿胀，呈空泡状，嵴断裂、缺失、缩短；电针组线粒体轻度肿胀，嵴结构较清晰。
结论电针足三里、三阴交、梁门可降低糖尿病胃轻瘫大鼠血糖，恢复胃动力，其机制可能与电针提高PDGF含量、改善线粒体超微结构有关。

Abstract:
ObjectiveTo observe the effect of electroacupuncture (EA) at the pressure points Zu San Li (ST36), San Yin Jiao (SP6) and Liang Men (ST21) on platelet-derived growth factor (PDGF) and the ultrastructure of mitochondria in rats with diabetic gastroparesis (DGP).
MethodsSixty Sprague Dawley (SD) rats were randomly separated into a normal control group (NC, n = 10) and a modeling group (n = 50). Rats in the modeling group received an injection of 2% streptozotocin (STZ) and a high-fat and high-glucose diet for eight weeks to establish a DGP rat model. At the same time, blood glucose and a general symptom score were recorded every week. After modeling, 30 successfully modeled rats were randomly separated into the following groups: the DGP group (n = 10), the EA group (n = 10) and the metoclopramide (MP) group (n = 10). After three weeks of intervention, the gastrointestinal propulsive rate was measured by measuring the optical density (OD). The concentration of Ca²⁺ was determined by fluorescence immunoassay, and levels of serum insulin (INS) and PDGF were determined by ELISA. The ultrastructure of mitochondria was observed with transmission electron microscopy.
Results(1) After intervention, levels of blood glucose and the general symptom score were greatly decreased in the EA group compared to the DGP group (P < 0.01). Compared with the DGP group, the gastric emptying rate and the intestinal propulsive rate of the EA group was significantly improved (P < 0.01), and there was no statistically significant difference between the EA and the NC groups. (2) Compared with the NC group, the levels of INS in the DGP group markedly decreased (P < 0.05), but there was no significant difference of INS levels between the EA and the MP groups. (3) Compared with the DGP group, the concentration of Ca²⁺ in the EA and the MP groups significantly increased (P < 0.01, P < 0.05, respectively). (4) Compared with the NC group, the average OD of PDGF in the DGP group was significantly higher (P < 0.01). Compared with the DGP group, levels of PDGF in the EA group increased significantly (P < 0.01). (5) There were abundant mitochondria with a clear structure and complete cristae in the NC group. However, in the DGP group, mitochondria were severely swollen, partly vacuolated, and cristae were either fractured, absent, or shortened. In the EA group, mitochondria were slightly swollen, with clear cristae.
ConclusionsElectroacupuncture at the points Zu San Li (ST36), San Yin Jiao (SP6) and Liang Men (ST21) may improve gastric motility in DGP by up-regulating the amount of PDGF and improving the ultrastructure of mitochondria.

HTML全文

参考文献(24)

施引文献

资源附件(0)